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Fast DNA Library Prep Kit

Categories NGS Library Construction
Brand Name: GDSBio
Model Number: K001-A,K001-B
Certification: CE, FDA,ISO9001,ISO13485
Place of Origin: China
MOQ: 1 bag
Payment Terms: L/C, D/A, D/P, T/T, Western Union,
Supply Ability: 10000 Box/Boxes per Day
Delivery Time: 8 work days
Packaging Details: small package or bulk distribute or OEM
Cat. No.: K001-A,K001-B
Specification: K001-A/24 rxns; K001-B/96 rxns; sample sack/ 6 rxns
Appearance: clear liquid
Stock: yes
Logo Printing: With Logo Printing
Transport Package: Packing
Production Capacity: 100 Bag/Bags per Day
Storage Conditions: -20°C
Sample Type: DNA
Sequencing Platform: Illumina
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Fast DNA Library Prep Kit

Fast DNA Library Prep Kit

【Product Name】

Fast DNA Library Prep Kit

【Cat. No./Spec.】

K001-A/24 rxns; K001-B/96 rxns; sample sack/ 6 rxns

【Product Description】

Aiming at Illumina high-throughput sequencing platform, this kit provides a convenient and universal DNA library construction scheme in one tube. It combines end repair and A-Tailing into one step, and reagents are highly premixed, greatly shortening the time of library construction and reducing the error caused by tedious steps. The end preparation, adapter ligation, amplification and purification of the fragmented double-stranded DNA can be performed within about 2 hours. Complete library quantification can be performed by dsDNA fluorescent dye method (e.g., Thermo Qubit Flex Fluorometer) or absolute quantification PCR after diluting the library to an appropriate concentration.

【Sample Type】

ApplicationSample TypeRecommended Amount
Whole genome sequencingHigh quality complex genomes50ng-1μg
Target capture sequencing of whole exomeHigh quality complex genomes10ng-1μg
Target capture sequencing of whole genomeFFPE DNA≥50ng
Target capture sequencing of whole genomecfDNA/ctDNA≥100pg
Whole genome sequencingMicrobial genome1ng-1μg
Whole genome sequencing(PCR-free)High quality DNA

≥50ng (no size selection)

≥200ng (size selection)

ChIP-SeqChIP DNA≥100pg
Targeted sequencingAmplicon≥100pg

【Storage Condition & Shelf Life】

All reagents should be stored at -20°C. Ligation Buffer is normal for crystals to precipitate at low temperatures, it should be balanced to room temperature before use. The product is valid for 12 months.

【Components】

Component24 rxns96 rxns
End Repair & A-Tailing Mix480 μl4×480 μl
Fast DNA Ligase120 μl2×240 μl
Fast Ligation Buffer600 μl4×600 μl
2× HIFI Library PCR Master Mix600 μl4×600 μl
Primer Mix*120 μl480 μl

* If there are more than one sample, #K002 and #K003 adapter primer mix is recommended. This kit provides a set of primers with index.

Note:recommended selection beads: #NC1011 GDSPure DNA Selection Magbeads or AMPure XP beads.


Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.


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