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MHC Class I C Peptide Elisa Kit Polypeptide Related Microtiter Plate

Categories Human ELISA Kit
Place of Origin: China
Brand Name: DLdevelop
Certification: CE, ISO9001:2015
MOQ: 48T
Price: Negotiation
Packaging Details: Carton Box
Delivery Time: 1-2 Working Days
Payment Terms: TT
Supply Ability: Abundant Supply
Quality guarantee period: "12 months "
Detection range: 2.5-4000pg/mL
Reactivity: ICB
Synonyms: MHC class I chain-related protein B
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    MHC Class I C Peptide Elisa Kit Polypeptide Related Microtiter Plate

    MHC Class I C Peptide Elisa Kit Polypeptide Related Microtiter Plate

    Traditional ELISA KitReady-to-Use ELISA KIT
    Product name:Human MHC Class I Polypeptide Related Sequence B (MICB) ELISA Kit
    Method:Sandwich
    Synonyms:

    MIC-B; PERB11.2; MHC class I chain-related protein B;

    Detection range:62.5-4000pg/mL
    Reactivity:MICB
    Size:96T/48T
    Quality guarantee period:for 12 months
    Catalog number:DL-MICB-Hu (traditional)DLR-MICB-Hu (ready-to-use)
    Assay length1-4.5Hours1-3.5Hours
    Advantages:
    • Competitive price.
    • High sensitivity.
    • High stability.
    • 12 months shelf life.
    • Pre-diluted Detection Reagent A and B
    • Reduction in the number of steps when conducting the test
    • All the reagents can be stored at 4℃
    • Faster reaction compare to other brands
    • 12 months shelf life
    Instruction Manual
    Introduction
    ItemStandardTest
    Description

    The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of MICB in human serum, plasma, tissue homogenates or other biological fluids.

    Conform
    IdentificationColorimetricPositive
    CompositionTraditional ELISA KitReady-to-Use ELISA KITConform
    Pre-coated, ready to use 96-well strip plate 1Pre-coated, ready to use 96-well strip plate 1
    Plate sealer for 96 wells 2Plate sealer for 96 wells 2
    Standard 2Standard 2
    Diluents buffer 1×45mLStandard Diluent 1×20mL
    Detection Reagent A 1×120LDetection Solution A 1×12mL
    Detection Reagent B 1×120LDetection Solution B 1×12mL
    TMB Substrate 1×9mLTMB Substrate 1×9mL
    Stop Solution 1×6mLStop Solution 1×6mL
    Wash Buffer (30concentrate) 1×20mLWash Buffer (30concentrate) 1×20mL
    Instruction manual 1Instruction manual 1
    Test principle

    The microtiter plate provided in this kit has been pre-coated with an antibody specific to MICB. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MICB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.

    After TMB substrate solution is added, only those wells that contain MICB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm10nm.

    The concentration of MICB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    Recovery

    Matrices listed below were spiked with certain level of recombinant MICB and the recovery rates were calculated by comparing the measured value to the expected amount of MICB in samples

    .

    MatrixRecovery range (%)Average(%)
    serum(n=5)83-10091
    EDTA plasma(n=5)81-10191
    heparin plasma(n=5)83-9890
    Linearity

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MICB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.


    Sample1:21:41:81:16
    serum(n=5)82-96%83-98%81-99%93-101%

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